Functional Characterization of Candidate Genes, Gohir.D05G103700 and Gohir.D12G153600, Identified through Expression QTL Analysis Using Virus-Induced Gene Silencing in Upland Cotton (Gossypium hirsutum L.)
نویسندگان
چکیده
Cotton (Gossypium spp.) is a major source of natural fiber and an important cash crop. The cotton growth habit architecture determine its productivity influence management strategies for commercial production. GATA transcription factors (TFs) control various developmental processes in plants, such as flower, bract embryo development, petal differentiation. As stable transformation still bottleneck many plant species, TRV-VIGS was used to manipulate gene expression different including Gossypium hirsutum L. In this study, we undertook the TRV-based VIGS functionally characterize two candidate genes, Gohir.D05G103700 Gohir.D12G153600, identified through QTL analysis five floral induction meristem identity genes using upland mini-core collection. Virus-induced silencing resulted up 1.4-fold reduction transcript level inoculated G3 G4, Gohir.D12G153600 2.3-fold single P05 relative mock-treated plant. TRV2-Gohir.D05G103700-inoculated plants G4 also exhibited loss supernumerary (fourth) squares, whereas TRV2-Gohir.D12G153600-inoculated did not show any observable phenotypic change plants. Altogether, study suggested that can be relatively rapidly, positive regulator indeterminate cotton, which could manipulated obtain with best suited cultivation area.
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ژورنال
عنوان ژورنال: Agriculture
سال: 2023
ISSN: ['2077-0472']
DOI: https://doi.org/10.3390/agriculture13051007